The Femto Bacterial DNA Quantification Kit can be used to detect and quantify bacterial DNA with high specificity and sensitivity. Bacterial DNA can be reliably quantified in a background of non-bacterial DNA such as fungal, animal, and plant DNA, etc. This is essential for downstream applications that require accurate DNA input including quantifying bacteria DNA template for Next-generation sequencing library preparation and metagenomic analysis amongst others. With the Femto Bacterial DNA Quantification Kit, one can dependably quantify as little as 20 fg DNA from 1 µl purified from biological liquids, bacterial cultures, or environmental DNA samples.